Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities.
Identifieur interne : 000398 ( Main/Exploration ); précédent : 000397; suivant : 000399Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities.
Auteurs : Patricia A. Rodriguez ; Remco Stam ; Tim Warbroek ; Jorunn I B. BosSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2014.
Descripteurs français
- KwdFr :
- Agrobacterium (physiologie), Animaux (MeSH), Aphides (génétique), Aphides (physiologie), Feuilles de plante (cytologie), Feuilles de plante (immunologie), Feuilles de plante (parasitologie), Feuilles de plante (physiologie), Immunité des plantes (MeSH), Interactions hôte-parasite (MeSH), Maladies des plantes (immunologie), Maladies des plantes (parasitologie), Marqueurs génétiques (génétique), Microscopie confocale (MeSH), Protéines d'insecte (génétique), Protéines d'insecte (métabolisme), Protéines de fusion recombinantes (MeSH), Réaction de polymérisation en chaine en temps réel (MeSH), Régulation de l'expression des gènes végétaux (génétique), Tabac (cytologie), Tabac (immunologie), Tabac (parasitologie), Tabac (physiologie), Transcription génétique (MeSH).
- MESH :
- cytologie : Feuilles de plante, Tabac.
- génétique : Aphides, Marqueurs génétiques, Protéines d'insecte, Régulation de l'expression des gènes végétaux.
- immunologie : Feuilles de plante, Maladies des plantes, Tabac.
- métabolisme : Protéines d'insecte.
- parasitologie : Feuilles de plante, Maladies des plantes, Tabac.
- physiologie : Agrobacterium, Aphides, Feuilles de plante, Tabac.
- Animaux, Immunité des plantes, Interactions hôte-parasite, Microscopie confocale, Protéines de fusion recombinantes, Réaction de polymérisation en chaine en temps réel, Transcription génétique.
English descriptors
- KwdEn :
- Agrobacterium (physiology), Animals (MeSH), Aphids (genetics), Aphids (physiology), Gene Expression Regulation, Plant (genetics), Genetic Markers (genetics), Host-Parasite Interactions (MeSH), Insect Proteins (genetics), Insect Proteins (metabolism), Microscopy, Confocal (MeSH), Plant Diseases (immunology), Plant Diseases (parasitology), Plant Immunity (MeSH), Plant Leaves (cytology), Plant Leaves (immunology), Plant Leaves (parasitology), Plant Leaves (physiology), Real-Time Polymerase Chain Reaction (MeSH), Recombinant Fusion Proteins (MeSH), Tobacco (cytology), Tobacco (immunology), Tobacco (parasitology), Tobacco (physiology), Transcription, Genetic (MeSH).
- MESH :
- chemical , genetics : Genetic Markers, Insect Proteins.
- cytology : Plant Leaves, Tobacco.
- genetics : Aphids, Gene Expression Regulation, Plant.
- immunology : Plant Diseases, Plant Leaves, Tobacco.
- chemical , metabolism : Insect Proteins.
- parasitology : Plant Diseases, Plant Leaves, Tobacco.
- physiology : Agrobacterium, Aphids, Plant Leaves, Tobacco.
- Animals, Host-Parasite Interactions, Microscopy, Confocal, Plant Immunity, Real-Time Polymerase Chain Reaction, Recombinant Fusion Proteins, Transcription, Genetic.
Abstract
Aphids are phloem-feeding insects that, like other plant parasites, deliver effectors inside their host to manipulate host responses. The Myzus persicae (green peach aphid) candidate effectors Mp10 and Mp42 were previously found to reduce aphid fecundity upon intracellular transient overexpression in Nicotiana benthamiana. We performed functional analyses of these proteins to investigate whether they activate defenses through similar activities. We employed a range of functional characterization experiments based on intracellular transient overexpression in N. benthamiana to determine the subcellular localization of Mp10 and Mp42 and investigate their role in activating plant defense signaling. Mp10 and Mp42 showed distinct subcellular localization in planta, suggesting that they target different host compartments. Also, Mp10 reduced the levels of Agrobacterium-mediated overexpression of proteins. This reduction was not due to an effect on Agrobacterium viability. Transient overexpression of Mp10 but not Mp42 activated jasmonic acid and salicylic acid signaling pathways and decreased susceptibility to the hemibiotrophic plant pathogen Phytophthora capsici. We found that two candidate effectors from the broad-host-range aphid M. persicae can trigger aphid defenses through different mechanisms. Importantly, we found that some (candidate) effectors such as Mp10 interfere with Agrobacterium-based overexpression assays, an important tool to study effector activity and function.
DOI: 10.1094/MPMI-05-13-0156-R
PubMed: 24006884
Affiliations:
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Le document en format XML
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Bos</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24006884</idno><idno type="pmid">24006884</idno><idno type="doi">10.1094/MPMI-05-13-0156-R</idno><idno type="wicri:Area/Main/Corpus">000438</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000438</idno><idno type="wicri:Area/Main/Curation">000438</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000438</idno><idno type="wicri:Area/Main/Exploration">000438</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities.</title><author><name sortKey="Rodriguez, Patricia A" sort="Rodriguez, Patricia A" uniqKey="Rodriguez P" first="Patricia A" last="Rodriguez">Patricia A. Rodriguez</name></author><author><name sortKey="Stam, Remco" sort="Stam, Remco" uniqKey="Stam R" first="Remco" last="Stam">Remco Stam</name></author><author><name sortKey="Warbroek, Tim" sort="Warbroek, Tim" uniqKey="Warbroek T" first="Tim" last="Warbroek">Tim Warbroek</name></author><author><name sortKey="Bos, Jorunn I B" sort="Bos, Jorunn I B" uniqKey="Bos J" first="Jorunn I B" last="Bos">Jorunn I B. Bos</name></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (physiology)</term><term>Animals (MeSH)</term><term>Aphids (genetics)</term><term>Aphids (physiology)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Genetic Markers (genetics)</term><term>Host-Parasite Interactions (MeSH)</term><term>Insect Proteins (genetics)</term><term>Insect Proteins (metabolism)</term><term>Microscopy, Confocal (MeSH)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (parasitology)</term><term>Plant Immunity (MeSH)</term><term>Plant Leaves (cytology)</term><term>Plant Leaves (immunology)</term><term>Plant Leaves (parasitology)</term><term>Plant Leaves (physiology)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Recombinant Fusion Proteins (MeSH)</term><term>Tobacco (cytology)</term><term>Tobacco (immunology)</term><term>Tobacco (parasitology)</term><term>Tobacco (physiology)</term><term>Transcription, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (physiologie)</term><term>Animaux (MeSH)</term><term>Aphides (génétique)</term><term>Aphides (physiologie)</term><term>Feuilles de plante (cytologie)</term><term>Feuilles de plante (immunologie)</term><term>Feuilles de plante (parasitologie)</term><term>Feuilles de plante (physiologie)</term><term>Immunité des plantes (MeSH)</term><term>Interactions hôte-parasite (MeSH)</term><term>Maladies des plantes (immunologie)</term><term>Maladies des plantes (parasitologie)</term><term>Marqueurs génétiques (génétique)</term><term>Microscopie confocale (MeSH)</term><term>Protéines d'insecte (génétique)</term><term>Protéines d'insecte (métabolisme)</term><term>Protéines de fusion recombinantes (MeSH)</term><term>Réaction de polymérisation en chaine en temps réel (MeSH)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Tabac (cytologie)</term><term>Tabac (immunologie)</term><term>Tabac (parasitologie)</term><term>Tabac (physiologie)</term><term>Transcription génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Genetic Markers</term><term>Insect Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Feuilles de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aphids</term><term>Gene Expression Regulation, Plant</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aphides</term><term>Marqueurs génétiques</term><term>Protéines d'insecte</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Insect Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines d'insecte</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Agrobacterium</term><term>Aphides</term><term>Feuilles de plante</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium</term><term>Aphids</term><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Host-Parasite Interactions</term><term>Microscopy, Confocal</term><term>Plant Immunity</term><term>Real-Time Polymerase Chain Reaction</term><term>Recombinant Fusion Proteins</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Immunité des plantes</term><term>Interactions hôte-parasite</term><term>Microscopie confocale</term><term>Protéines de fusion recombinantes</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Aphids are phloem-feeding insects that, like other plant parasites, deliver effectors inside their host to manipulate host responses. The Myzus persicae (green peach aphid) candidate effectors Mp10 and Mp42 were previously found to reduce aphid fecundity upon intracellular transient overexpression in Nicotiana benthamiana. We performed functional analyses of these proteins to investigate whether they activate defenses through similar activities. We employed a range of functional characterization experiments based on intracellular transient overexpression in N. benthamiana to determine the subcellular localization of Mp10 and Mp42 and investigate their role in activating plant defense signaling. Mp10 and Mp42 showed distinct subcellular localization in planta, suggesting that they target different host compartments. Also, Mp10 reduced the levels of Agrobacterium-mediated overexpression of proteins. This reduction was not due to an effect on Agrobacterium viability. Transient overexpression of Mp10 but not Mp42 activated jasmonic acid and salicylic acid signaling pathways and decreased susceptibility to the hemibiotrophic plant pathogen Phytophthora capsici. We found that two candidate effectors from the broad-host-range aphid M. persicae can trigger aphid defenses through different mechanisms. Importantly, we found that some (candidate) effectors such as Mp10 interfere with Agrobacterium-based overexpression assays, an important tool to study effector activity and function. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24006884</PMID><DateCompleted><Year>2014</Year><Month>04</Month><Day>03</Day></DateCompleted><DateRevised><Year>2015</Year><Month>07</Month><Day>08</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>27</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Mp10 and Mp42 from the aphid species Myzus persicae trigger plant defenses in Nicotiana benthamiana through different activities.</ArticleTitle><Pagination><MedlinePgn>30-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-05-13-0156-R</ELocationID><Abstract><AbstractText>Aphids are phloem-feeding insects that, like other plant parasites, deliver effectors inside their host to manipulate host responses. The Myzus persicae (green peach aphid) candidate effectors Mp10 and Mp42 were previously found to reduce aphid fecundity upon intracellular transient overexpression in Nicotiana benthamiana. We performed functional analyses of these proteins to investigate whether they activate defenses through similar activities. We employed a range of functional characterization experiments based on intracellular transient overexpression in N. benthamiana to determine the subcellular localization of Mp10 and Mp42 and investigate their role in activating plant defense signaling. Mp10 and Mp42 showed distinct subcellular localization in planta, suggesting that they target different host compartments. Also, Mp10 reduced the levels of Agrobacterium-mediated overexpression of proteins. This reduction was not due to an effect on Agrobacterium viability. Transient overexpression of Mp10 but not Mp42 activated jasmonic acid and salicylic acid signaling pathways and decreased susceptibility to the hemibiotrophic plant pathogen Phytophthora capsici. We found that two candidate effectors from the broad-host-range aphid M. persicae can trigger aphid defenses through different mechanisms. Importantly, we found that some (candidate) effectors such as Mp10 interfere with Agrobacterium-based overexpression assays, an important tool to study effector activity and function. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rodriguez</LastName><ForeName>Patricia A</ForeName><Initials>PA</Initials></Author><Author ValidYN="Y"><LastName>Stam</LastName><ForeName>Remco</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Warbroek</LastName><ForeName>Tim</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Bos</LastName><ForeName>Jorunn I B</ForeName><Initials>JI</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/J005258/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005819">Genetic Markers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019476">Insect Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001042" MajorTopicYN="N">Aphids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005819" MajorTopicYN="N">Genetic Markers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006790" MajorTopicYN="N">Host-Parasite Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019476" MajorTopicYN="N">Insect Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D057865" MajorTopicYN="Y">Plant Immunity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>4</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24006884</ArticleId><ArticleId IdType="doi">10.1094/MPMI-05-13-0156-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Bos, Jorunn I B" sort="Bos, Jorunn I B" uniqKey="Bos J" first="Jorunn I B" last="Bos">Jorunn I B. Bos</name><name sortKey="Rodriguez, Patricia A" sort="Rodriguez, Patricia A" uniqKey="Rodriguez P" first="Patricia A" last="Rodriguez">Patricia A. Rodriguez</name><name sortKey="Stam, Remco" sort="Stam, Remco" uniqKey="Stam R" first="Remco" last="Stam">Remco Stam</name><name sortKey="Warbroek, Tim" sort="Warbroek, Tim" uniqKey="Warbroek T" first="Tim" last="Warbroek">Tim Warbroek</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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